Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Pseudomonas putida is a soil bacterium that is frequently found in sites polluted with toxic chemicals. It is able to survive in these sites because of its ability to tolerate high levels of endogenous and exogenous oxidative stress. But, how? The answer to this question remains largely unknown.

A study, recently published in The ISME Journal, aims at shedding light on the ultimate reason of such phenotypic property. For this, metabolic network-wide routes for NADPH generation were inspected when Pputida KT2440 was challenged with a sub-lethal H2O2 dose as a proxy of oxidative conditions. 

It was seen that periplasmic glucose processing was rerouted to cytoplasmic oxidation, and the cyclic operation of the pentose phosphate pathway led to significant NADPH-forming fluxes, exceeding biosynthetic demands by ~50%. The resulting NADPH surplus, in turn, fueled the glutathione system for H2O2 reduction.

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