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Multiple-site diversification of regulatory sequences enables interspecies operability of genetic devices
The features of the light-responsive cyanobacterial CcaSR regulatory module that determine interoperability of this optogenetic device between Escherichia coli and Pseudomonas putida have been examined.
Pseudomonas putida in the quest of programmable chemistry
Traditional microbial biotechnology is in the midst of a deep transformation brought about by conceptual and technical breakthroughs, as well as by the major change
Standards: the building blocks of complexity
Standards. The building blocks of complexity, is the latest issue of the Mètode journal, and it focuses on standards in science, with special emphasis on
Recombination-independent genome editing through CRISPR/Cas9-enhanced TargeTron delivery
Group II introns are retroelements (elements that are transcribed into RNA, reverse-transcribed into DNA and then inserted into a new site in the genome) that
Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida
Pseudomonas putida has many naturally-evolved metabolic and stress-tolerant properties, which makes this organism ideal for many biotechnological applications. Glucose catabolism, a redox reaction that allows to
A simple workflow to identify, clone and quantify the function of recombinases in Gram-negative bacteria
The endogenous RecA homology-based recombination system has been typically used to edit the bacterial chromosome in Gram-negative bacteria, and although this technique is efficient, it