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Standardization of biological parts for S. cerevisiae
Emma Wattsa, Leonardo Rios Solisa,b* aCentre for Synthetic and Systems Biology (SynthSys), University of Edinburgh, King’s Buildings, Edinburgh, United Kingdom bInstitute for Bioengineering, School
DNA-BOT: A low-cost, automated DNA assembly platform for synthetic biology
Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology, and the ability to explore a genetic design space
Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene
The mismatch repair (MMR) system is one of the key molecular devices that prokaryotic cells have for ensuring fidelity of DNA replication. While the canonical
Digitalizing heterologous gene expression in Gram‐negative bacteria with a portable ON/OFF module
While prokaryotic promoters controlled by signal‐responding regulators typically display a range of input/output ratios when exposed to cognate inducers, non-naturally occurring cases are known to
Multiple-site diversification of regulatory sequences enables interspecies operability of genetic devices
The features of the light-responsive cyanobacterial CcaSR regulatory module that determine interoperability of this optogenetic device between Escherichia coli and Pseudomonas putida have been examined.
Pseudomonas putida in the quest of programmable chemistry
Traditional microbial biotechnology is in the midst of a deep transformation brought about by conceptual and technical breakthroughs, as well as by the major change