News
Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene
The mismatch repair (MMR) system is one of the key molecular devices that prokaryotic cells have for ensuring fidelity of DNA replication. While the canonical
Digitalizing heterologous gene expression in Gram‐negative bacteria with a portable ON/OFF module
While prokaryotic promoters controlled by signal‐responding regulators typically display a range of input/output ratios when exposed to cognate inducers, non-naturally occurring cases are known to
Multiple-site diversification of regulatory sequences enables interspecies operability of genetic devices
The features of the light-responsive cyanobacterial CcaSR regulatory module that determine interoperability of this optogenetic device between Escherichia coli and Pseudomonas putida have been examined.
Pseudomonas putida in the quest of programmable chemistry
Traditional microbial biotechnology is in the midst of a deep transformation brought about by conceptual and technical breakthroughs, as well as by the major change
Standards: the building blocks of complexity
Standards. The building blocks of complexity, is the latest issue of the Mètode journal, and it focuses on standards in science, with special emphasis on
Recombination-independent genome editing through CRISPR/Cas9-enhanced TargeTron delivery
Group II introns are retroelements (elements that are transcribed into RNA, reverse-transcribed into DNA and then inserted into a new site in the genome) that
Compartmentalization in membrane-surrounded organelles has the potential to overcome obstacles associated with the engineering of metabolic pathways. In this work, endoplasmic reticulum-derived vesicles loaded with enzymes of a metabolic pathway are used. https://pubs.acs.org/doi/10.1021/acssynbio.0c00241
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