In the implementation of Boolean logic circuits in cells, the focus is mainly on the genetic components, although it must be taken into account that the context in which the circuit performs is crucial for its outcome.
In this recent work, 20 genetic NOT logic gates have been characterised in up to 7 bacterial-based contexts (combinations of four plasmid backbones and three bacterial hosts, two Escherichia coli and one Pseudomonas putida), generating a total of 135 different functions.
Interestingly, each gate showed seven different dynamic behaviours depending on the context. In other words, gates can be fine-tuned by changing only contextual parameters, and the effect of the genetic background on synthetic constructs expands functionality.
Access the full article, published in Nature Communications, here.