Engineering and standardization of posttranscriptional biocircuitry in Saccharomyces cerevisiae

Synthetic biology is largely based on transcriptional circuitry, in which promoters, terminators and transcription factor sites are utilized to build devices and modules. However, biocircuitry can also be engineered using RNA and/or protein components involved in posttranscriptional steps of gene expression. For example, messenger RNA translation can be modulated by targeting the 5’-untranslated region using … Leer más

Modulating transcription through development of semi-synthetic yeast core promoters

Saccharomyces cerevisiae can be used as a platform the production of bulk and fine chemicals, although for this it is necessary to introduce heterologous metabolic pathways and fine-tune gene expression. The latter can be done by regulating transcription, for example by altering the gene’s promoter or terminator. Eukaryotic promoters are usually composed of a core … Leer más

CCCtag – a standardized designed set of coiled-coil heterodimerizing modules for tunable regulation of localization, transcriptional activation and light- and chemical signal based regulation

Protein-protein interactions are involved in almost all cellular processes, they define scaffolds, regulate cellular localization and participate in functioning in cellular machines, such as those performing transcription, replication, splicing and others. In order to manipulate cellular processes we need a reliable standardized set of orthogonal protein-protein interaction modules.  Coiled-coil peptide heterodimers are small polypeptide modules … Leer más