Standardsinsybio

Round Table on SynBio & Education – December 3rd 2020

Join us on December 3rd between 4 and 5:30 pm (CET) for a virtual round table discussion on synthetic biology and education. The speakers on our panel will be: Jake Beal, University of Iowa, USA. He is a Senior Scientist at BBN Technologies and  a research affiliate of the University of Iowa. Previously, he was … Leer más

Unblocking the bottleneck – our do’s and don’ts for faster, more successful DNA outsourcing

Authors: Alan Walker & Davide De Lucrezia For much of its history, the exponential decline in the cost of DNA has been held as the central metric of progress in Synthetic Biology. Often compared to Moore’s law, it has sparked a wealth of startups and new research. But cost is an incomplete metric that hides … Leer más

Round Table on standards in SynBio: views of the BioTech industry

The “BioRoboost Round Table: views of the BioTech Industry” took place on October 1st. Participants were able to discuss many topics, including the most urgent standardisation needs of the BioTech industry. The event was divided into two main sessions. During the first part, the project coordinator shared an introduction of the BioRoboost project, followed by … Leer más

Capturing Multicellular System Designs Using Synthetic Biology Open Language (SBOL)

Synthetic biology aims to develop novel biological systems and increase their reproducibility using engineering principles such as standardisation and modularisation. It is important to represent and share these systems in a standard way to ensure that they can be easily understood, reproduced, and used by other researchers. The Synthetic Biology Open Language (SBOL) is a … Leer más

For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!

The role of synthetic biology as a fuel for the bioeconomy requires clarification of the terms typically adopted by this growing scientific-technical field. In particular, the concept of ‘chassis’ as a defined, reusable biological frame where non-native components can be plugged in and out to create new functionalities lies at the boundary between frontline bioengineering … Leer más

Naked Bacterium: Emerging Properties of a Surfome-Streamlined Pseudomonas putida Strain

Environmental bacteria are usually equipped with native surface-attachment elements that can hinder the engineering of biofilms. In this work, the genome of Pseudomonas putida KT2440 was engineered to strip the cells of most of their outer-facing structures, involved in motion, binding to surfaces and biofilm formation. In particular, 23 segments were deleted, resulting in a … Leer más

Standardization of biological parts for S. cerevisiae

Emma Wattsa, Leonardo Rios Solisa,b*    aCentre for Synthetic and Systems Biology (SynthSys), University of Edinburgh, King’s Buildings, Edinburgh, United Kingdom  bInstitute for Bioengineering, School of Engineering, University of Edinburgh, King’s Buildings, Edinburgh, United Kingdom * Corresponding author:  Dr Leonardo Rios Solis (E -mail: Leo.Rios@ed.ac.uk; Telephone: +441316513561) As our understanding of genetic circuits has improved, … Leer más

DNA-BOT: A low-cost, automated DNA assembly platform for synthetic biology

Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology, and the ability to explore a genetic design space by building extensive libraries of DNA constructs is essential for creating programmed biological systems. With multiple DNA assembly methods and standards adopted in the Synthetic Biology community, automation of the … Leer más

Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene

The mismatch repair (MMR) system is one of the key molecular devices that prokaryotic cells have for ensuring fidelity of DNA replication. While the canonical MMR of E . coli involves 3 proteins (encoded by mutS , mutL and mutH), the soil bacterium Pseudomonads putida has only 2 bona fide homologues (mutS and mutL ) … Leer más

Digitalizing heterologous gene expression in Gram‐negative bacteria with a portable ON/OFF module

While prokaryotic promoters controlled by signal‐responding regulators typically display a range of input/output ratios when exposed to cognate inducers, non-naturally occurring cases are known to have an OFF state of zero transcription (as ideally needed for synthetic circuits). To overcome this problem, we have modelled and implemented a simple digitalizer module that completely suppresses the … Leer más