Standardsinsybio

How information is difficult to capture in the form of standards – WP5 interview fragments

A selection of fragments of interviews published inWP5 are made available in this document. In the interviews the participants describe how information is difficult to capture in the form of standards and they refer to different types of contexts: biological, socio-cultural and technical to explain why that is the case. Biological contexts Biology 1/Emergence «So, … Leer más

The microbial terroir: open questions on the Nagoya protocol applied to microbial resources

The Nagoya Protocol on Access and Benefit-sharing, primarily designed for vascular plant and animal resources, is also extended to the use of microbial resources, but its application to the microbiological realm has raised many doubts and provoked criticisms. This is because of the particularities of microbial ecology and the technical and legal difficulties encompassed in its … Leer más

Engineering and standardization of posttranscriptional biocircuitry in Saccharomyces cerevisiae

Synthetic biology is largely based on transcriptional circuitry, in which promoters, terminators and transcription factor sites are utilized to build devices and modules. However, biocircuitry can also be engineered using RNA and/or protein components involved in posttranscriptional steps of gene expression. For example, messenger RNA translation can be modulated by targeting the 5’-untranslated region using … Leer más

Sqrutiny sketcher

The question of orthogonality is inherent to the endeavor of creating standards in Synthetic Biology, although few systematic approaches have been proposed yet. In a first step, gene circuits will inevitably be designed, experimentally tested and characterized in one bacterial species, e.g. one of the model organisms as Escherichia coli. In order to use the … Leer más

Engineered bacteria as an early-stage drug discovery platform against diseases caused by protein misfolding and aggregation

Protein misfolding and aggregation are common pathological features for many human diseases, such as Alzheimer’s disease, type 2 diabetes, amyotrophic lateral sclerosis and cancer. Aiming to increase the effectiveness of early drug discovery programs for these conditions, the lab of Dr. Georgios Skretas has developed a bacterial platform that enables the biosynthesis of molecular libraries … Leer más

Engineering a Thermophilic Microbial Chassis for Synthetic Biology Applications

The OMIC-Engine Athens Hub is working on incorporating the advantages of high-temperature biocatalysis into Synthetic Biology (SB) applications. Led by the Enzyme and Microbial Biotechnology Unit at the Department of Biology of the National and Kapodistrian University of Athens (PI Prof. Dimitris Hatzinikolaou) and in collaboration with the Laboratory of Biotechnology the National Technical University … Leer más

Refactoring the Conjugation Machinery of Promiscuous Plasmid RP4

Chromosomal exchange and subsequent recombination of the cognate DNA between bacteria was one of the most useful genetic tools (e.g., Hfr strains) for genetic analyses of E. coli before the genomic era. In this paper, yeast assembly has been used to recruit the conjugation machinery of environmentally promiscuous RP4 plasmid into a minimized, synthetic construct that enables … Leer más