Engineered bacteria as an early-stage drug discovery platform against diseases caused by protein misfolding and aggregation

Protein misfolding and aggregation are common pathological features for many human diseases, such as Alzheimer’s disease, type 2 diabetes, amyotrophic lateral sclerosis and cancer. Aiming to increase the effectiveness of early drug discovery programs for these conditions, the lab of Dr. Georgios Skretas has developed a bacterial platform that enables the biosynthesis of molecular libraries … Leer más

Engineering a Thermophilic Microbial Chassis for Synthetic Biology Applications

The OMIC-Engine Athens Hub is working on incorporating the advantages of high-temperature biocatalysis into Synthetic Biology (SB) applications. Led by the Enzyme and Microbial Biotechnology Unit at the Department of Biology of the National and Kapodistrian University of Athens (PI Prof. Dimitris Hatzinikolaou) and in collaboration with the Laboratory of Biotechnology the National Technical University … Leer más

Refactoring the Conjugation Machinery of Promiscuous Plasmid RP4

Chromosomal exchange and subsequent recombination of the cognate DNA between bacteria was one of the most useful genetic tools (e.g., Hfr strains) for genetic analyses of E. coli before the genomic era. In this paper, yeast assembly has been used to recruit the conjugation machinery of environmentally promiscuous RP4 plasmid into a minimized, synthetic construct that enables … Leer más

When biology became engineering: adopting standards for living systems

Synthetic Biology, born in the early 2000s, aims to take biotechnology one step further by introducing, into the biological realm, conceptual frameworks from a range of engineering disciplines, including electrical, computer and industrial engineering. The goal is to be able to create complex biological objects and, for this, it is necessary to design and construct … Leer más

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Pseudomonas putida is a soil bacterium that is frequently found in sites polluted with toxic chemicals. It is able to survive in these sites because of its ability to tolerate high levels of endogenous and exogenous oxidative stress. But, how? The answer to this question remains largely unknown. A study, recently published in The ISME Journal, … Leer más

Modelling co-translational dimerization for programmable nonlinearity in synthetic biology

Nonlinearity is fundamental in the performance of natural and synthetic biological networks, as many key functional motifs in microbial systems rely on nonlinear molecular dynamics. Nevertheless, the rational design of nonlinearity is an unmet challenge due to the lack of mathematical modelling that accounts for the mechanistic basis of nonlinearity. In this study, a model … Leer más

Quantitative assessment of morphological traits of planktonic bacterial aggregates

Planktonic flocs composed of multiple bacterial strains can be used biocatalytic agents in aqueous media for industrial applications and environmental cleanup. Microbial consortia are characterized by including division of labour among the different microbial species that compose the biofilm, allowing to perform complex biochemical transformations. But this division of labour is not the only think … Leer más

An automated DIY framework for experimental evolution of Pseudomonas putida

Adaptive laboratory evolution is a general and effective strategy for optimizing the design of engineered genetic circuits and upgrading metabolic phenotypes. However, the specific characteristics of each microorganism typically require the use of exclusive conditions that must be adjusted to the biological chassis at stake. In this work, a do‐it‐yourself (DIY) approach has been used … Leer más

A standardized inverter package borne by broad host range plasmids for genetic circuit design in Gram-negative bacteria

Genetically encoded logic gates (and, in particular, inverters or NOT gates) are the main building blocks for designing circuits, engineering biosensors, or decision-making devices in synthetic biology. Nevertheless, the repertoire of inverters readily available for different species is limited. In this work, a variety of NOT gates were recreated as broad host range constructs assembled … Leer más