Type I CRISPR inhibitors from phage: phage-borne anti-CRISPR genes that encode protein inhibitors of the type I-F CRISPR-Cas system of Pseudomonas aeruginosa.
A platform of genetic codes comprising fewer than 20 amino acids was created by removing specific amino acids from E. coli S30 cell-free reaction mixture to eliminate the targeted endogenous translation pathways connecting the specific amino acids and the codons. The tRNA variant with the altered anticodon loop was then added, to reassign Ala or Ser to the unassigned codons.
Simple cells (SimCells) whose native chromosomes were removed by digestion of heterologous nuclease and replaced by synthetic genetic circuits of interest.
A version control system for cell engineering that integrates a new cloud-based version control software for cell lines’ digital footprint tracking and molecular barcoding of living samples.
Adding unique DNA sequence as barcode for new genetic modification done in microbes
The number of codons used to encode the canonical amino acids have been reduced, through the genome-wide substitution of target codons by defined synonyms. A variant of E. coli genome (strain Syn61) has been created with size of 4 Mb synthetic genome (based on strain MSD42) through a high-fidelity convergent total synthesis.
The codons encoded PV1 protein of poliovirus have been recoded using deoptimized codon pair, leading to 631 mutations, resulting in “death by a thousand cut” attenuation.
Constructing viral genome variants containing synthetic replacements of the capsid coding sequences either by deoptimizing synonymous codon usage (PV-AB) or by maximizing synonymous codon position changes of the existing wild-type poliovirus codons.
Biocontainment Finder is a free resource supporting the search and retrieval of biological containment strategies with the aim of improving biosafety. The Biocontainment Finder database contains more than 40 citations and summaries of peer-reviewed literature. The summary of each containment strategy is composed of its description, features, already tested organisms, efficiency (if any), proposed/tested applications, and plausible concerns. While the reference of published strategy is provided, it does not include full text journal articles; however, links to the full text can be easily retrieved from PubMed.
Citations in Biocontainment Finder primarily stem from the corrections in PubMed. For those biological circuits which have been proposed or tested but lack of peer-reviewed will not be included in this database. If interested, they can be found in the iGEM website for the registry of standard parts. The contents of this database have been last updated in July 2021. New contents with biocontainment relevant will not be included afterwards.
Before start to search for a biocontainment strategy, you should get to know the general biosafety measurements. Biosafety measurements are composed of policies, rules, and procedures to define how one should work in various facilities to handle microbiological agents such as bacteria, viruses, parasites, fungi, and other related agents including microbiological products. Key elements of a biosafety measurement include institutional biosafety review, facilities (infrastructure and personal protection equipment), training programs, surveillances, and emergency response plans.
You can use filters to narrow your search results either by the filter: choose one of the eight strategies alone, or by combination of strategy and keyword. To apply a filter, click the filter you would like to activate from the sidebar. To start new search, click the main bar “Biocontainment Finder” to return to the starting setting.
ERA Environmental Release application
ERA- I intended (application)
ERA- M measured
ERA- N not intended
ERA- Y yes but not measured
GMO Genetically Modified Organism
NA Not Applicable
NK Not Known
RG Risk Group
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