A standardized inverter package borne by broad host range plasmids for genetic circuit design in Gram-negative bacteria

Genetically encoded logic gates (and, in particular, inverters or NOT gates) are the main building blocks for designing circuits, engineering biosensors, or decision-making devices in synthetic biology. Nevertheless, the repertoire of inverters readily available for different species is limited.

In this work, a variety of NOT gates were recreated as broad host range constructs assembled in low, medium, and high copy number plasmid backbones of the SEVA collection. This inverter package (20 different versions of 12 distinct gates) borne by BHR plasmids is a useful toolbox for the design of genetic circuits in a variety of Gram-negative species.

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